Geoff Parling is set to make his first Test start for England in Saturday's 6 Nations clash with Wales after Tom Palmer was cut from the squad.
Leicester lock Parling is understood to have been promoted into the starting line-up along with Northampton scrum-half Lee Dickson and Scarlets number eight Ben Morgan.
All three made an impact off the bench in England's opening Six Nations victories over Scotland and Italy.
Palmer started both those games but he has been released by interim head coach Stuart Lancaster to play for Stade Francais this weekend.
Northampton lock Courtney Lawes is set to provide the second-row cover on the bench after overcoming a knee injury.
Manu Tuilagi and Toby Flood are also back in England's match-day squad for the first time in the tournament, with both Leicester players expected to start from the bench as Owen Farrell and Charlie Hodgson keep their places.
As a result, Harlequins full-back Mike Brown and centre Jordan Turner-Hall have dropped out of England's 22-man squad.
Lancaster has also released Harlequins hooker Chris Brooker, Northampton flanker Calum Clark, Wasps scrum-half Joe Simpson and Leicester number eight Thomas Waldrom.
Lancaster said: "We've had a great two days of training and some difficult selection decisions with players coming back into the equation.
"There are some players who have missed out this time but their attitude in training has been outstanding.
"We will look forward to meeting again on Wednesday night with our match day 22 and finalising preparations for the game."
Palmer is the biggest casualty and perhaps a victim of England not enjoying the lineout solidity they would have liked over the opening two rounds of the championship.
"We were nine from 11 in Scotland and 14 from 17 in Italy, with sleet all over the ball against a very competent Italian pack," said forwards coach Graham Rowntree.
"Not all of it was clean so I am looking to improve our delivery and certainly the tempo at which we go about our line-out."
Lancaster will confirm his line-up to face Wales on Thursday morning.
Parling, who is expected to run the lineout, toured with England to Australia in 2010 but he returned home with a neck problem and then suffered a succession of knee injuries which kept him out of England contention until this season.
Dickson and Morgan have been in line for a promotion since their game-changing impact against Italy in Rome last week, when both players injected a much-needed tempo to England's performance.
Ben Youngs has been struggling for form and is set to be named on the bench while Phil Dowson will cover all three back row positions.
While Dickson brings a zip and an energy to England's game, Morgan's powerful ball-carrying has impressed the management since his arrival in the national squad.
Given Morgan plys his club trade for the Scarlets, he has also been able to pass on some useful tips about the Welsh players.
"He knows those players. Ben's got his own game to worry about. He has made giant strides with us in terms of his game development," Rowntree said.
"He's a very coachable man. He showed that coming off the bench in Italy in all parts of his game. I have been really impressed with him."
England will go into the game without any specialist cover at wing or full-back, although Tuilagi could be pressed into action out wide if necessary.
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